v1.3.0 · Live
SPLISOFORMSSplicing Proteomics Knowledgebase
Translating alternative splicing into structural, immunological, and regulatory insights — across cancer types and tissues.
Structure Explorer
AlphaFold 3 models for every long-read isoform — per-residue pLDDT confidence and interactive Mol* visualization.
Splicing Impact
How splice variants reshape the fold: DSSP secondary-structure shifts, solvent-accessibility changes, and IUPred3 disorder — scored per residue against the canonical.
Drug Pockets
AlphaFold 3 co-folded drug–protein complexes. Track whether each binding pocket is preserved, disrupted, or lost in every splice isoform.
Neoepitope Atlas
Splice-junction neoepitopes scored by a three-tool MHC ensemble (MHCflurry · NetMHCpan-4.2 · BigMHC) and ranked into P1–P4 priority tiers.
Functional Domains
Pfam HMM mapping and TED structural segmentation, classifying every domain as gained, lost, or truncated relative to the reference protein.
Protein Interactions
STRING seven-channel interaction evidence, plus AlphaFold-derived homodimer and heterodimer interface disruption — quantifying which contacts a splice event breaks.
NMD & Transcript Fate
Nonsense-mediated decay prediction via the 50-nucleotide rule, with ORF-completeness classification and transcript-fate annotation for every novel isoform.
PTM Accessibility
How splicing reshapes post-translational modification sites — ΔSASA exposure shifts for phosphorylation, ubiquitination, and acetylation, gated by structural confidence.
Multi-Tissue Coverage
Long-read transcriptomes from ccRCC organoids and breast-cancer tissue, anchored to the GENCODE v47 / GRCh38 reference proteome.